Western Blot Positive WB detected in: NIH/3T3 whole cell lysate, Rat brain tissue, Mouse heart tissue All lanes: NDUFB8 antibody at 2μg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 22, 21, 19 kDa Observed band size: 22 kDa
IHC image of CSB-PA015655LA01HU diluted at 1:400 and staining in paraffin-embedded human heart tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
IHC image of CSB-PA015655LA01HU diluted at 1:400 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Supplier Page from CUSABIO Technology LLC for NDUFB8 Antibody